THREE DIMENSIONAL BIOCOMPATIBLE SCAFFOLDS FOR EX-VIVO EXPANSION AND TRANSPLANTATION OF STEM CELLS

摘要

A method of transplanting undifferentiated hematopoietic stem cells into a recipient is disclosed. The method comprising (a) expanding the undifferentiated hematopoietic stem cells to increase the number of the hematopoietic stem cells by (i) culturing in a stationary phase plug-flow bioreactor stromal cells under continuous flow of a culture medium on a three dimensional biocompatible substrate to thereby generate a three dimensional stromal cell culture; and (ii) seeding undifferentiated hematopoietic stem cells into the stationary phase plug-flow bioreactor including the three dimensional stromal cell culture and under a continuous flow of a culture medium, thereby expanding the undifferentiated hematopoietic stem cells and obtaining a three dimensional stromal cell culture comprising increased number of hematopoietic stem cells; and (b) transplanting the three dimensional stromal cell culture comprising hematopoietic stem cells resulting from (a) into the recipient.