发明名称 |
Nucleic acid construct containing full length genome of human hepatitis C virus, recombinant full length virus genome-replicating cells having the nucleic acid construct transferred thereinto and method of producing hepatitis C virus particle |
摘要 |
The present invention provides a method for replicating efficiently an RNA containing fulllength HCV genomic sequence and a method for producing HCV virus particles containing fulllength HCV replicon RNA or fulllength HCV genomic RNA by using a cell culture system. Further, the present invention relates to a method for producing hepatitis C virus particles which comprises culturing a cell, into which a replicon RNA comprising a nucleotide sequence comprising a fulllength genomic RNA sequence of hepatitis C virus of the genotype 2a, at least one selectable marker gene and/or at least one reporter gene and at least one IRES sequence or the fulllength genomic RNA of hepatitis C virus of the genotype 2a is introduced, and generating virus particles in the culture medium. Still further the present invention relates also to a hepatitis C vaccine and an antibody against hepatitis C virus particles.
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申请公布号 |
US2010047896(A1) |
申请公布日期 |
2010.02.25 |
申请号 |
US20090583465 |
申请日期 |
2009.08.21 |
申请人 |
TOKYO METROPOLITAN ORGANIZATION FOR MEDICAL RESEARCH;TORAY INDUSTRIES, INC. |
发明人 |
WAKITA TAKAJI;KATO TAKANOBU;DATE TOMOKO;MIYAMOTO MICHIKO;TANABE JUN-ICHI;SONE SABURO |
分类号 |
C12N7/00;A61K35/76;A61P37/04;C07K16/08;C07K16/10;C12N1/15;C12N1/19;C12N1/21;C12N5/00;C12N7/01;C12N15/63;C12Q1/02;C12Q1/68;G01N33/15;G01N33/50;G01N33/53;G01N33/567;G01N33/576 |
主分类号 |
C12N7/00 |
代理机构 |
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地址 |
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