| 摘要 |
Disclosed is a rapid dual purpose PCR based method for identifying two or more pathogens in a sample, such as a stool or environmental (soil, water) sample, including Giardia and/or Cryptosporidium in a single real-time PCR reaction. This is of particular utility in the screening and detection of pathogen(s) in water, soil, and/or other environmental applications, as well as in stool sample testing/screening. The present methods are more sensitive than conventional ELISA or IFA microscopic bead methods of detection. The present methods have utility in the detection/screening of these and other pathogens in human an non-human (veterinary and environmental) applications. An internal control construct (ICC) for use in the PCR based nucleic acid detector method is also disclosed. |
