发明名称 |
NOVEL NUCLEIC ACID DERIVATIVE, AND METHOD FOR PREPARING NUCLEASE-RESISTANT NUCLEIC ACID BY USING SAME |
摘要 |
<p>Disclosed is a method for preparing a nuclease-resistant nucleic acid, which comprises the steps of mixing a nucleic acid composed of at least three nucleotides, a terminal deoxynucleotide transferase and a 2',4'-crosslinked nucleoside triphosphate with one another and incubating the resulting mixture. Examples of the 2',4'-crosslinked nucleoside triphosphate to be used in the method include 5-methyl-2'-O,4'-C-(methyleneoxy- methylene)uridine-5'-triphosphate, 5-methyl-2'-O,4'-C- (aminomethylene)uridine-5'-triphosphate and 5-methyl-2'-O,4'-C-(benzyloxymethylene)uridine-5'- triphosphate. According to the method, it becomes possible to impart excellent nuclease resistance to an existing functional nucleic acid afterward in a simple manner.</p> |
申请公布号 |
WO2010021344(A1) |
申请公布日期 |
2010.02.25 |
申请号 |
WO2009JP64522 |
申请日期 |
2009.08.19 |
申请人 |
KUWAHARA, MASAYASU;JAPAN SCIENCE AND TECHNOLOGY AGENCY;OBIKA, SATOSHI |
发明人 |
KUWAHARA, MASAYASU;OBIKA, SATOSHI |
分类号 |
C07H19/20;C12N15/09;C12P19/34;C07H19/10 |
主分类号 |
C07H19/20 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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