| 摘要 |
<p>The present invention relates to methods for identifying and optionally quantifying stained cells in a cell or tissue sample and determining expression level of a target, as well as methods for determining staining intensity and/or staining quality of a stained sample, calibrating digital imaging apparatus using the methods, as well as systems and a computer readable mediums therefore. In particular the invention relates to a method for identifying stained targets in a sample and uses of said method, said method comprising providing at least one digital representation of the sample wherein the sample has been stained with a stain staining a target, and filtering the digital representation with at least three filters, each of said filters comprising filter constants), applying said filter constant(s) to each pixels in at least a subset of the digital representation and determining an eigensolution based on the filter output for each pixel in the subset, wherein said filters are capable of enhancing the stained targets obtaining a filtered representation, segmenting the digital representation based on information from the optionally normalized filtered representation into stained cell targets and background, optionally combining with the digital representation, thereby obtaining a segmented representation, wherein said segmented representation discriminates between stained targets and other structures in the digital representation, and from said segmented representation identifying the stained targets in the sample.</p> |
