发明名称 |
METHODS FOR PURIFYING ANTIBODIES USING PROTEIN A AFFINITY CHROMATOGRAPHY |
摘要 |
<p>This invention provides a method for purifying a monomeric monoclonal antibody which comprises contacting the sample, wherein the sample comprises the monomeric monoclonal antibody, host cell impurities, dimers, and higher order aggregates, with a Protein A affinity chromatography column; eluting the monomeric monoclonal antibody from the Protein A affinity chromatography column with an elution buffer; and collecting one or more fractions of the monomeric monoclonal antibody to form a Protein A product pool, wherein the product pool comprises less than 5% higher order aggregate, and has a pH from about 3.2 to about 4.5, thereby purifying the monomeric monoclonal antibody from the sample. This invention also provides a method for purifying a monomeric monoclonal antibody which comprises eluting with acetate or citrate, optionally in the presence of amino acids. This invention also provides a method for purifying a monomeric monoclonal antibody which comprises conducting the method within certain temperature ranges.</p> |
申请公布号 |
WO2010019493(A1) |
申请公布日期 |
2010.02.18 |
申请号 |
WO2009US53260 |
申请日期 |
2009.08.10 |
申请人 |
MERCK SHARP & DOHME CORP.;CHMIELOWSKI, REBECCA;GREEN-TREXLER, ERIN;ROUSH, DAVID |
发明人 |
CHMIELOWSKI, REBECCA;GREEN-TREXLER, ERIN;ROUSH, DAVID |
分类号 |
C07K1/22;C07K16/06 |
主分类号 |
C07K1/22 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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