METHOD FOR SACCHARIFICATION PRETREATMENT OF HERBACEOUS BIOMASS

摘要

PROBLEM TO BE SOLVED: To provide a method for saccharification pretreatment of herbaceous biomass which sufficiently removes lignin of herbaceous biomass and facilitates a cellulose saccharification reaction caused by an enzyme. SOLUTION: Herbaceous biomass is inoculated with Phanerochaete flavido-alba and the Phanerochaete flavido-alba is cultured together with the herbaceous biomass to decompose lignin of the herbaceous biomass. The herbaceous biomass is sterilized at 80-120°C and supplied for the culture. The herbaceous biomass is supplied for the culture in a water content of 70-90 wt.%. The culture is carried out at 20-35°C. The culture is carried out at a pH of 2.0-7.0. Phanerochaete flavido-alba is cultured and then the herbaceous biomass is treated by wet grinding. The herbaceous biomass is supplied for the wet grinding treatment in a water content of not less than 70 wt.%. The wet grinding treatment is performed by using a disc mill. COPYRIGHT: (C)2010,JPO&INPIT