METHOD FOR DETECTION AND/OR QUANTIFICATION OF A NUCLEIC ACID

摘要

The present invention relates to a method of detection-quantification and analysis of nucleic acids having at least one primer labelled at its 5' extremity with a fluorophore. Said primer presents, in the bases adjacent to the label, at least three guanines or three cytosines. These bases modify the fluorescent signal and said modification is utilised for detection of the nucleic acid. The method of the invention may provide a stage of analysis of denaturation curves such as to permit identification of a specific sequence of nucleic acids, its application consequently being indicated in analysis, genotyping and detection of pathogens, among others.