发明名称 |
COMPOSITION COMPRISING HIGH CONCENTRATION OF BIOLOGICALLY ACTIVE MOLECULES AND PROCESSES FOR PREPARING THE SAME |
摘要 |
Large scale processes for producing high purity samples of biologically active molecules of interest from bacterial cells are disclosed. The methods comprise the steps of producing a lysate solution by contacting a cell suspension of said plurality of cells with lysis solution; neutralizing said lysate solution with a neutralizing solution to produce a dispersion that comprises neutralized lysate solution and debris; filtering the dispersion through at least one filter; performing ion exchange separation on said neutralized lysate solution to produce an ion exchange eluate; and performing hydrophobic interaction separation on said ion exchange eluate to produce a hydrophobic interaction solution. Further, provided are compositions comprising large scale amounts of plasmid DNA produced by the disclosed large scale processes. |
申请公布号 |
EP2153260(A1) |
申请公布日期 |
2010.02.17 |
申请号 |
EP20080756211 |
申请日期 |
2008.05.23 |
申请人 |
VGX PHARMACEUTICALS, INC. |
发明人 |
DRAGHIA-AKLI, RUXANDRA;HEBEL, HENRY;CAI, YING |
分类号 |
G02B6/293 |
主分类号 |
G02B6/293 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|