发明名称 |
PEPTIDE STANDARDS |
摘要 |
<p>The invention relates to methods for making a peptide standard for mass spectrometry said method comprising (a) identifying endopeptidase cleavage sites in a parent polypeptide sequence of interest; (b) selecting peptide sequences from said parent polypeptide which are defined by endopeptidase cleavage sites of step (a); (c) adding a C-terminal extension to each selected sequence; wherein if the endopeptidase cleavage site is C-terminal to its recognition sequence then the C-terminal extension comprises 1 to 6 amino acids, wherein if the endopeptidase cleavage site is N-terminal to its recognition sequence then the C-terminal extension comprises said recognition sequence, wherein if the endopeptidase cleavage site is within its recognition sequence then the C-terminal extension comprises the remainder of said recognition sequence C-terminal to the cleavage site; and (d) synthesizing a peptide having the extended amino acid sequence of step (c). The invention also relates to peptide standards and methods of analysing samples.</p> |
申请公布号 |
EP2152727(A1) |
申请公布日期 |
2010.02.17 |
申请号 |
EP20080750514 |
申请日期 |
2008.05.02 |
申请人 |
KING'S COLLEGE LONDON;GUY'S AND ST. THOMAS' NHS FOUNDATION TRUST |
发明人 |
TURNER, CHARLES;DALTON, RAYMOND, NEIL |
分类号 |
C07K14/76;C07B59/00;C07K7/08;G01N33/68 |
主分类号 |
C07K14/76 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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