发明名称 |
Gene silencing using mRNA-cDNA hybrids |
摘要 |
The present invention provides novel compositions and methods for suppressing the expression of a targeted gene using mRNA-cDNA duplexes. The invention further provides novel methods and compositions for generating amplified mRNA-cDNA hybrids, whose quantity is high enough to be used for the invention's gene silencing transfection. This improved RNA-polymerase chain reaction method uses thermocycling steps of promoter-linked double-stranded cDNA or RNA synthesis, in vitro transcription and then reverse transcription to amplify the amount of mRNA-cDNA hybrids up to two thousand folds within one round of the above procedure.
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申请公布号 |
US7662791(B2) |
申请公布日期 |
2010.02.16 |
申请号 |
US20010920342 |
申请日期 |
2001.08.01 |
申请人 |
UNIVERSITY OF SOUTHERN CALIFORNIA |
发明人 |
LIN SHI-LUNG;CHUONG CHENG-MING;WIDELITZ RANDALL B. |
分类号 |
A61K48/00;C12N15/10 |
主分类号 |
A61K48/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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