发明名称 |
Fluorescent detection method and reagent |
摘要 |
Disclosed is a method for increasing the fluorescence of a Cyanine dye molecule comprising at least one NO2 group characterized by the reduction of the at least one NO2 group to NHOH or NH2 by the action of a nitroreductase. The cyanine dye molecule comprising at least one NO2 group can be used as a substrate for detecting nitroreductase enzyme activity in a composition and allows for the use of a nitroreductase enzyme in an enzyme-reporter system for the detection of analytes, binding reactions or gene expression.
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申请公布号 |
US7662973(B2) |
申请公布日期 |
2010.02.16 |
申请号 |
US20020182996 |
申请日期 |
2002.10.16 |
申请人 |
GE HEALTHCARE UK LIMITED |
发明人 |
THOMAS NICHOLAS;MICHAEL NIGEL PAUL;MILLAR VALERIE;DAVIES BETH;BRIGGS MARK SAMUEL JONATHAN |
分类号 |
C09B7/02;G01N33/533;C09B23/00;C09B23/08;C09B67/22;C09K11/07;C12P17/12;C12Q1/26;G01N21/78;G01N33/535;G01N33/543;G01N33/84 |
主分类号 |
C09B7/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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