ASSAY FOR PEPTIDE HORMONE RECEPTOR LIGAND AND ITS USE

摘要

<P>PROBLEM TO BE SOLVED: To provide a method for determining whether a candidate compound is a non-peptide agonist of a peptide hormone receptor or not. Ž<P>SOLUTION: A candidate compound is brought into contact with a form of the peptide hormone receptor which has an enhanced ability to amplify the intrinsic activity of a non-peptide agonist. The second messenger signaling activity of the enhanced receptor is measured in the presence of the candidate compound and compared to the second messenger signaling activity of the enhanced receptor measured in the absence of the candidate compound. A change in second messenger signaling activity indicates that the candidate compound is an agonist. An increase in second messenger signaling activity indicates that the compound is either a full or partial agonist, and a decrease in second messenger signaling activity indicates that the compound is an inverse agonist. Ž<P>COPYRIGHT: (C)2010,JPO&INPIT Ž