| 摘要 |
Systems and methods for enhancing quantitative transfer of analytes to an adsorptive substrate are provided. A preferred embodiment provides an improved transfer system and method that reduces "blow through" of low molecular weight analytes and increases the adsorption of low molecular weight, for example proteins of about 30 kDa or less compared to conventional transfer techniques. It has been discovered that using a secondary porous backing membrane in conjunction with an adsorptive membrane such as nitrocellulose or polvinylidene fluoride increases the efficiency of analyte interaction with the adsorption membrane compared to transfers techniques using the adsorption membrane without the secondary backing membrane. This improvement of quantitative analyte transfer efficiency facilitates a wider range of detection processes, such as direct analysis by MALDI mass spectrometry than in earlier applications of the process without the secondary backing membrane.
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