发明名称 |
Method and kit for determination of prostacyclin in plasma |
摘要 |
A solid-phase immunoassay for 6-keto-Prostaglandin F1alpha, the stable hydrolysis product of prostacyclin (Prostaglandin I2) is disclosed. Prostacyclin, a potent vasodilator with anti-platelet and anti-proliferative properties is an effective treatment for primary pulmonary hypertension and pulmonary arterial hypertension associated with scleroderma and scleroderma-like syndrome. Levels of 6-keto-Prostaglandin F1alpha can be directly correlated with levels of prostacyclin. Therefore, 6-keto-Prostaglandin F1alpha has become the indicator of choice to measure prostacyclin levels. The single step immunoassay for 6-keto-Prostaglandin F1alpha uses the bioluminescent protein, aequorin as a label. Analyte-label conjugates were constructed by linking the carboxyl group of 6-keto-Prostaglandin F1alpha and lysine residues of aequorin by chemical conjugation methods. The binding properties of 6-keto-Prostaglandin F1alpha towards its antibody and the bioluminescent properties of aequorin are retained in the conjugate. The concentration of 6-keto-Prostaglandin F1alpha after extraction from plasma shows good correlation with the concentration of 6-keto-Prostaglandin F1alpha obtained without prior extraction of the same plasma sample. The assay allows the measurement of 6-keto-Prostaglandin F1alpha directly in plasma without any pre-treatment of the samples, which results in a much simpler method with a faster assay time.
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申请公布号 |
US7659078(B1) |
申请公布日期 |
2010.02.09 |
申请号 |
US20030620806 |
申请日期 |
2003.07.17 |
申请人 |
UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION |
发明人 |
DAUNERT SYLVIA;POON MICHAEL;DESAI URVEE;DEO SAPNA K. |
分类号 |
G01N33/53;C07K16/18;C07K16/26;C12Q1/66;G01N33/537;G01N33/543;G01N33/88 |
主分类号 |
G01N33/53 |
代理机构 |
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