发明名称 |
Method and compositions for obtaining mature dendritic cells |
摘要 |
We describe an improved method for generating sizable numbers of mature dendritic cells from nonproliferating progenitors in human blood. The first step or "priming" phase is a culture of T cell depleted mononuclear cells in medium supplemented with GM-CSF and IL-4 to produce immature dendritic cells. The second step or "differentiation" phase requires the exposure to dendritic cell maturation factor such as monocyte conditioned medium. Using this two-step approach, substantial yields are obtained. The dendritic cells derive from this method have all the features of mature cells. They include a stellate cell shape, nonadherence to plastic, and very strong T cell stimulatory activity. The mature dendritic cells produced according to this invention are useful for activating T cells.
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申请公布号 |
US7659119(B2) |
申请公布日期 |
2010.02.09 |
申请号 |
US19960600483 |
申请日期 |
1996.02.12 |
申请人 |
ARGOS THERAPEUTICS, INC.;THE ROCKEFELLER UNIVERSITY |
发明人 |
STEINMAN RALPH M.;BHARDWAJ NINA;SCHULER GEROLD |
分类号 |
C12N5/02;A61K38/00;C12N5/0784 |
主分类号 |
C12N5/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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