METHOD FOR ROBUST CONTROL OVER A SOLUABLE FACTOR MICROENVIRONMENT WITHIN A THREE-DIMENSIONAL GEL MATRIX

摘要

A method is provided of generating a gradient within gel matrix received in a channel of a microfluidic device. A source reservoir in communication with the input of the channel is filled with a first fluid. A sink reservoir in communication with the output of the channel is filled with a second fluid. A soluble factor is deposited in the source reservoir such that the soluble factor diffuses into the channel and forms the gradient. The soluble factor in source reservoir is replenished to maintain the gradient in a generally pseudo-steady state and the second fluid in the sink reservoir is replaced.