THE METHOD FOR PREPARING PATTERNED PROTEIN NANOARRAYS USING A SELF-ASSEMBLED BLOCK COPOLYMER THIN FILM AND THE PATTERNED PROTEIN NANOARRAYS THEREBY

摘要

PURPOSE: A method for preparing a protein nano array having pattern using self-assembled block copolymer thin film is provided to selectively adsorb a protein on self-assembled block copolymer thin film. CONSTITUTION: A method for preparing a protein nano array having pattern comprises: a step(step 1) of spin-coating a random copolymer which is dissolved in solvent to form neutral surface layer; a step(step 2) of spin-coating a block copolymer on the neutral surface layer to apply; a step of performing thermal treatment of the block copolymer to induce self-assembly and prepare template having regular structure; a step(step 4) of immersing the template in a protein solution to prepare protein nano array on which protein is adsorbed; and a step(step 5) of washing the protein nano array to form nano pattern. The random copolymer is polystyrene-polybenzocyclobutene-polymethylmethacrylate(P(S-r-BCB-r-MMA)). The protein of step 4 is a functionalized protein which is able to perform target specific bond.