A METHOD OF RECOVERING HIGHLY PURIFIED VWF OR FACTOR VIII/VWF-COMPLEX

摘要

Disclosed is a method of recovering highly purified vWF or factor VIII/vWF-complex by means of immunoaffinity chromatography, which is characterized in that vWF or factor VIII/vWF-complex bound to an immunoadsorbent is eluted by means or an eluting agent containing as an essential active ingredient a zwitterion, while retaining the molecular integrity of vWF or of the factor VIII/vWF-complex. Furthermore, the invention relates to a preparation containing highly purified vWF having a specific activity of at least 100 U/mg protein, obtainable by immunoaffinity chromatography, as well as to the use of this preparation for producing a medicament for the treatment of vWD or of phenotypic hemophilia A.