发明名称 |
Methods for high-throughput and quantitative proteome analysis |
摘要 |
The invention provides methods for identifying and quantifying polypeptides in a sample. The methods include the steps of labeling peptides in a polypeptide sample with an isotope tag; adding a plurality of peptide standards to the polypeptide sample, wherein the peptide standards are labeled with an isotopically distinct version of the isotope tag; resolving the labeled sample and standard peptides into a plurality of fractions; analyzing the resolved fractions using mass spectrometry; identifying an isotope-tagged sample peptide in an analyzed fraction; and determining the amount of the identified isotope-tagged sample peptide in the analyzed fraction by comparison to the amount of isotope tagged standard peptide in the same fraction.
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申请公布号 |
US7655433(B2) |
申请公布日期 |
2010.02.02 |
申请号 |
US20030455246 |
申请日期 |
2003.06.04 |
申请人 |
THE INSTITUTE FOR SYSTEMS BIOLOGY |
发明人 |
AEBERSOLD RUDOLF H. |
分类号 |
C12Q1/37;C12N9/48;C12N9/76;C12P19/30;C12Q1/00;C12Q1/34;G01N33/543;G01N33/60;G01N33/68 |
主分类号 |
C12Q1/37 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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