| 摘要 |
Disclosed is a composition comprising: (a) a virus-like particle (VLP) comprising coat proteins, mutants or fragments thereof, of an RNA-bacteriophage with at least one first attachment site, wherein said VLP is recombinantly produced by a host, and said VLP is essentially free of host RNA, and wherein said mutant or said fragment of said coat protein of said RNA-bacteriophage is capable of assembling into a VLP, and wherein said mutant of said coat protein of said RNA-bacteriophage has an amino acid sequence identity of at least 80% with the amino acid sequence of said coat protein, and wherein said fragment of said coat protein of said RNA-bacteriophage is a polypeptide of at least 70% of the length of said coat protein (b) at least one antigen with at least one second attachment site; wherein said at least one antigen (b) is linked to said VLP (a) through said at least one first attachment site and said at least one second attachment site, and wherein said composition further comprises at least one polyanionic macromolecule packaged inside said VLP, and wherein said polyanionic macromolecule is selected from the group consisting of: (a) polyanionic polypeptides; (b) polyanionic saccharides; (c) polyanionic organic polymers; and (d) nucleic acids, wherein said nucleic acid is not a toll-like receptor ligand.
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