MUTANT WXOD GENE, ITS NUCLEOTIDE SEQUENCE, MUTANT STRAIN INCREASING BIOSYNTHESIS OF XANTHAN GUM, METHOD FOR PREPARING THE SAME AND PROCESS FOR PRODUCING XANTHAN GUM BY USING THE SAME

摘要

PURPOSE: A mutant strain which enables xanthan gum containing a mutant gene is provided to massively produce xanthan gum under the conventional technique and installation. CONSTITUTION: A mutant wxoD gene which is related to xanthan gum synthesis is derived from Xanthomonas oryzae strain. The Xanthomonas oryzae strain is Xanthomonas oryzae pv. Oryzae(KACC 10331) strain. The mutant gene wxoD is mutated by inserting the transposon. The mutant gene wxoD contains sequence of sequence number 1. A method for preparing the Xanthomonas sp.(deposit number: KACC91351) comprises: a step of adding transposon to the Xanthomonas sp.; a step of searching the mutant wxo gene using a specific primer; and a step of measuring biosynthesis rate of the xanthangum in the mutant strain and selecting.