MARKER GENES BASED ON AMIODARONE AND CARBAMAZEPINE TREATMENT FOR SCREENING OF DRUG INDUCING PULMONARY TOXICITY AND SCREENING METHOD USING THE SAME

摘要

PURPOSE: A marker gene for screening drug causing pulmonary toxicity and a screening method using the same are provided to monitor and determine drug or chemical. CONSTITUTION: A marker gene for screening drug causing pulmonary toxicity contains gene of which expression by drug. The drug causing pulmonary toxicity is amiodarone and carbamazepine. A DNA microarray chip for detecting the drug contains oligonucleotide containing partial or whole marker gene sequence. A method for screening the drug causing pulmonary toxicity comprises: a step of treating test chemical to human bronchial epithelial cells; a step of isolating RNA from chemical-treated cells as an experimental group and untreated cells as a control group; a step of synthesizing RNA with the experimental group and control group; a step of labeling the cDNA with other fluorescence having other colors; a step of analyzing DNA microarray; and a step of comparing expression level.