PSEUDOMONAS FLUORESCENS STRAINS FOR PRODUCTION OF EXTRACELLULAR RECOMBINANT PROTEIN

摘要

Provided herein are compositions and methods for improving secretion of heterologous protein from a population of Pseudomonas fluorescens-derived cells. The compositions include host cell populations that have been genetically modified to increase secretion into the extracellular space of a heterologous protein of interest. The genetically-modified host cell populations are useful for improving the extracellular secretion of any protein of interest, including therapeutic proteins, hormones, a growth factors, extracellular receptors or ligands, proteases, kinases, blood proteins, chemokines, cytokines, antibodies and the like. In various embodiments, the modified P. fluorescens host cell populations include one or more genomic mutations responsible for or contributing to the improved level of extracellular secretion. The host cell populations of the invention include the modified P. fluorescens strains deposited in the American Type Tissue Culture Collection and assigned accession numbers PTA-8981 and PTA-8982.