| 摘要 |
<p>Substrates for thrombin and assays for determining the level of bioactive thrombin in a sample are disclosed. The substrate has the general formula: A-X-Z-A' wherein one of either A or A' comprises a luminescent chelate, for example a lanthanide chelate. The other one of A or A' comprises a first partner of a binding pair, for example Biotin and optionally a spacer. X forms a tri or tetra peptide, for example X'-Phe-Aze-Arg, X'-Phe-Pip-Arg and X'-Phe-Pro-Arg, wherein X is absent or selected among Lys, Ahx, lie, and VaI. Z comprises a linker, for example a diamine linker.</p> |
