| 摘要 |
A microscope apparatus 1 comprises: an illumination optical system illuminating a sample with laser light from laser light sources 32, 42; a fluorescence detection optical system that detects fluorescence from the sample; a plurality of fluorescence cubes 61 that are provided on an optical path of the illumination optical system and leads the laser light to the sample; and an objective lens 16. At least one of the fluorescence cube includes an optical member 60 that makes a principal ray of the laser light substantially parallel to an optical axis of the illumination optical system and concentrates the laser light on a given position that is on a pupil position P of the objective lens and separates from the optical axis, thereby providing a microscope apparatus capable of changing from a confocal microscope to a total-internal-reflection fluorescence microscope by exchanging a fluorescence cube used in the fluorescence microscope.
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