发明名称 |
tdcBC/pckA GENE-INACTIVATED MICROORGANISM AND METHOD OF PRODUCING L-THREONINE USING THE SAME |
摘要 |
The present invention provide a microorganism comprising an inactivated chromosomal tdcBC gene and an inactivated chromosomal pckA gene, which has remarkably improved productivity of L-threonine. Also, the present invention provides a method of producing L-threonine using the microorganism. The microorganism is prepared by incorporating by a recombination technique an antibiotic resistance gene into a pckA gene on the chromosome of a bacterial strain containing an L-threonine degradation-associated operon gene, tdcBC, which is inactivated. The microorganism has the effect of preventing degradation and intracellular influx of L-threonine due to the inactivation of the tdcBC operon gene, and includes more activated pathways for L-threonine biosynthesis. Therefore, the microorganism is useful for mass production of L-threonine because of being capable of producing L-threonine in high levels and high yields even in the presence of high concentrations of glucose. |
申请公布号 |
EP1611232(B1) |
申请公布日期 |
2010.01.20 |
申请号 |
EP20040725555 |
申请日期 |
2004.04.02 |
申请人 |
CJ CHEILJEDANG CORPORATION |
发明人 |
PARK, YOUNG HOON;LEE, BYOUNG CHOON;KIM, DAE C.;LEE, JIN HO;CHO, JAE YONG |
分类号 |
C12N1/21;C12N9/88;C12P13/08 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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