REACTEUR A ENZYME

摘要

1356283 Enzymatic reactions MONSANTO CO 19 April 1971 [18 March 1970 4 Feb 1971] 24428/71 Headings C2C and C2S [Also in Divisions G1 and C3] Glucose lactic acid benzoyl tyrosine, gluconic acid, a mixture of fructose and glucose, and tosyl arginine are prepared by an enzymatic reaction using a through-flow reactor for enzymatic reactions comprising a macroporous reactor core characterized by a polymeric surface having adsorption-promoting nitriles, acid amido or urethane groups and an active enzyme adsorbed thereon and cross-linked in place by a cross-linking agent. In the examples using (a) a polyacrylonitrile coated polyethylene core: glucose is prepared from starch using amyloglucosidase and saccharifying amylase (15), (22); lactic acid is prepared by passing phosphoenol through a first disc on which is adsorbed pyruvate kinase producing pyruvate which then reacts with the lactase dehydrogenase immobilized on a second disc to form the lactic acid (17); benzoyl tyrosine is prepared from the reaction of benzoyl tyrosine p-nitroanilide with immobilized chymotrypsin (19); gluconic acid is prepared from the reaction of glucose with immobilized glucose oxidase (21); and a mixture of fructose and glucose is prepared from the reaction of glucose with immobilized glucose isomerase (24); and (b) a polycaprolactam core: tosyl arginine is prepared from the reaction of tosyl arginine methyl ester and trypsin (29).