MULTIPLEX QUANTIFICATION OF NUCLEIC ACIDS IN DISEASED CELLS

摘要

A process for assessing the tendency of a compound to induce peripheral neuropathy or peripheral lipodystrophy that includes contacting nucleic acid from a host cell that has been treated with the compound with and amplification reaction mixture that contains at least two primers and/or probes that provide detectable signals during a polymerase chain reaction, wherein the first primer and/or probe provides a detectable signal on the occurrence of the transcription of host mitochondrial nucleic acid; and the second primer and/or probe provides a second detectable signal on the occurrence of the transcription of host nuclear nucleic acid.