| 摘要 |
<p>A device and a method for performing isoelectric focussing of sample compounds in a liquid sample is disclosed. The device comprises a multi-compartment frame (105) with at least two adjacent compartments (106A-106E), each compartment being adapted to contain a liquid phase, adjacent compartments being in fluidic contact with one another via fluidic connections (108A-108D) formed in separation walls (107A-107D). The compartments are placed on top of an immobilized pH gradient gel (100) in a gel strip reception (102) thereby establishing a fluidic contact between the gel and the liquid phases contained in the compartments, and providing a pH gradient that extends into the liquid phases in the compartments. The device further comprises at least two electrodes (109,110) for applying an electric field across the compartments, wherein under influence of the electric field, a respective sample compound is forced to move via the fluidic connections (108A-108D) in the pH gradient until an isoelectric focussing of the respective sample compound in one of the compartments (106A-106E) is accomplished. A semipermeable layer (104) prevents the sample compounds from migrating into the pH gradient gel. In a second embodiment, instead of using a semipermeable membrane to prevent macromolecules from penetrating into the gel, the mesh size of the gel is reduced.</p> |
