RESPIRATORY INFECTIOUS PATHOGEN DIFFERENTIAL DIAGNOSIS AND SIMULTANEOUS ANTIBIOTICS RESISTANCE ANALYSIS, KIT AND CHIP COMPRISING SAME

摘要

PURPOSE: A multiplex kit and chip for determining pathogen analysis and antibiotics resistance are provided to accurately confirm pathogen of respiratory infection and reduce antibiotics resistance rate. CONSTITUTION: A method for analyzing pathogen diagnosis of infectious diseases and antibiotics resistance comprises: a step of designing a chip in which probe of amino modifier or thiol modifier is spotted; a step of isolating bacteria genomic DNA, virus RNA and DNA from a sample; a step of amplifying probe target site of each pathogen and probe target site for antibiotics resistance analysis through multiplex RT-PCR or multiplex PCR; a step of preparing the target DNA PCR product containing fluorescence dye; a step of hybridizing and washing; and a step of analyzing pathogen diagnosis and antibiotics resistance.