A BIOMARKER BASED ON METHOTREXATE TREATMENT FOR SCREENING OF DRUG INDUCING TERATOGENICITY AND SCREENING METHOD USING THEREOF

摘要

PURPOSE: A biomarker for screening teratogenicity-inducing chemical is provided to increase or decrease gene expression by methotrexate treatment and monitor chemical. CONSTITUTION: A gene expression of biomarker for screening teratogenicity-inducing chemical is increased or decreased by treating with a drug. The drug is methotrexate. A DNA microarray chip for screening the chemical comprises oligonucleotide containing whole or partial biomarker gene or its complementary oligonucleotide. A method for screening the chemical comprises: a step of treating test compound to human placenta-derived cells; a step of isolating RNA from the compound-treated experimental group and control group; a step of synthesizing cDNA and labeling the experimental group and control group with different color fluorescence; a step of hybridizing cDNA with DNA microarray chip; a step of analyzing DNA microarray chip; and a step of comparing experimental group with the control group.