The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.
申请公布号
WO2010004265(A1)
申请公布日期
2010.01.14
申请号
WO2009GB01679
申请日期
2009.07.06
申请人
OXFORD NANOPORE TECHNOLOGIES LIMITED;JAYASINGHE, LAKMAL;BAYLEY, HAGAN;CHELEY, STEPHEN;MCKEOWN, BRIAN;WHITE, JAMES;CLARKE, JAMES
发明人
JAYASINGHE, LAKMAL;BAYLEY, HAGAN;CHELEY, STEPHEN;MCKEOWN, BRIAN;WHITE, JAMES;CLARKE, JAMES