发明名称 |
Randomized dna libraries and double-stranded rna libraries, use and method of production thereof |
摘要 |
This invention relates to DNA libraries based on plasmid or viral vectors that can express double-stranded RNA of 10-30 base pairs in length with all possible sequences, where each of the double stranded RNA is formed by a single RNA molecule in the form of hairpin, or formed by two separate RNA molecules with different 3'-overhangs. Each single member in such a DNA library encodes all components of a double stranded RNA as specified above. Such a library can be used in screening for double stranded RNA species that can induce a given phenotype without prior knowledge of their target genes. This invention further relates to a method to generate such a DNA library.
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申请公布号 |
US2010009856(A1) |
申请公布日期 |
2010.01.14 |
申请号 |
US20030517324 |
申请日期 |
2003.06.23 |
申请人 |
SINOGENOMAX SOMPANY LTD. |
发明人 |
LIANG ZICAI;ZHANG HONG-YAN;CHENG MEIHONG;SHEN YAN |
分类号 |
C12N15/09;C40B30/00;C07H21/02;C07H21/04;C12N15/10;C12N15/11;C12Q1/68;C40B40/08 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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