PSEUDOMONAS PUTIDA STYRENE MONOOXYGENASE VARIANTS

摘要

The present invention provides isolated polynucleotides derived from Pseudomonas putida, and encoding polypeptides having styrene monooxygenase activity. The polynucleotides of the present invention are derived from the styAB gene from Pseudomonas putida CA-3, using error prone PCR-based in vitro evolution. The polypeptides encoded by these polynucleotides are useful as biocatalysts for the synthesis of oxygenated compounds, as they exhibit improved properties, such as enzymatic activity and enantiospecificity. Methods for obtaining oxygenated compounds are also provided, as are organisms that express the polypeptides and are thus useful for carrying out said biocatalytic syntheses.