A PURIFIED RECOMBINANT BATROXOBIN WITH HIGH SPECIFIC ACTIVITY

摘要

A purified recombinant batroxobin with high specific activity, which has the following properties: (a) the batroxobin has a molecular weight of 29-32kDa; (b) at least 90% of the batroxobin have 6 pairs of disulfide bonds which correctly match at Cys 7 -Cys 139 , Cys 26 -Cys 42 , Cys 74 -Cys 230 , Cys 118 -Cys 184 , Cys 150 -Cys 163 and Cysl 174 -Cys 199 ; (c) positions 146 and 225 in SEQ ID NO:1 are modified as N-glycosylation; and (d) the specific activity of the batroxobin is equal to or greater than 1500KU/mg protein.