发明名称 |
NMR detection of foreign PAS domain ligands |
摘要 |
Specific binding of a foreign core ligand to a PAS domain, wherein the PAS domain is predetermined, prefolded in its native state, and comprises a hydrophobic core that has no NMR-apparent a priori formed ligand cavity, is determined using NMR spectra of the PAS domain in the presence and absence of a foreign ligand bound within the hydrophobic core. A functional surface binding specificity of a PAS domain is changed by (a) introducing into the hydrophobic core of the PAS domain a foreign ligand of the PAS domain; and (b) detecting a change in the functional surface binding specificity of the PAS domain.
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申请公布号 |
US7645569(B2) |
申请公布日期 |
2010.01.12 |
申请号 |
US20030677733 |
申请日期 |
2003.10.01 |
申请人 |
BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM |
发明人 |
GARDNER KEVIN H.;AMEZCUA CARLOS A.;ERBEL PAULUS J. A.;CARD PAUL B. |
分类号 |
C12Q1/00;C12N9/12;C12Q1/48;G01N33/53 |
主分类号 |
C12Q1/00 |
代理机构 |
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主权项 |
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地址 |
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