发明名称 |
METHODS FOR THE RECOMBINANT PRODUCTION OF ANTIFUSOGENIC PEPTIDES |
摘要 |
A process for the recombinant production of an antifusogenic peptide by expression of a nucleic acid encoding said antifusogenic peptide as repeat peptide in a microbial host cell, isolation of inclusion bodies containing said repeat peptide, solubilization of the inclusion bodies, and isolation of the antifusogenic peptide after cleavage, characterized by washing the inclusion bodies with a denaturing agent at a pH value of or below pH 6.5, solubilizing said inclusion bodies containing the repeat peptide at a pH value of at least pH 9 and cleaving sa id repeat peptide to obtain said antifusogenic peptide.
|
申请公布号 |
CA2443365(C) |
申请公布日期 |
2010.01.12 |
申请号 |
CA20032443365 |
申请日期 |
2003.10.08 |
申请人 |
F. HOFFMAN - LA ROCHE AG |
发明人 |
SCHANTZ, CHRISTIAN;SEEBER, STEFAN;KOPETZKI, ERHARD;KACZMAREK, ALEXANDRA |
分类号 |
C12N15/09;C12N15/62;A61K38/00;A61K38/16;C07K14/08;C07K14/155;C07K14/16;C07K19/00;C12N15/33;C12N15/48;C12N15/49;C12N15/63;C12P21/00;C12P21/02 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|