AN ASSAY FOR SCREENING/RANKING ANTIOXIDANTS BASED ON THEIR EFFECTS ON MEMBRANE STEROL LATERAL ORGANIZATION

摘要

A method of screening an antioxidant for potency and/or toxicity in vitro, the method includes contacting the antioxidant with a model system comprising a sterol superlattice formation capable of generating a detectible signal, wherein the detectible signal changes in a parameter representative of an integrity of the sterol superlattice formation; and detecting and/or measuring disruption of the sterol superlattice formation, wherein the disruption is caused by oxidation of sterol upon contacting sterol with a prooxidant and the antioxidant and thereby screening the antioxidant for potency and/or toxicity.