METHOD FOR PREPARING TAGATOSE USING MICROORGANISMS WHICH GALACTOSE UPTAKE ARE REPRESSED

摘要

PURPOSE: A method for producing tagatose using microorganism in which use of galactose is suppressed is provided to obtain tagatose without separate galactose isolation step. CONSTITUTION: A method for preparing recombinant microorganism which produces tagatose comprises: a step of modificating gene to remove a gene which relates to inflow of galactose into the cell a step of designing vector for overexpression of arabinose isomerase from the microorganism; and a step of transforming the vector to the gene-modified microorganism. The microorganism is Escherichia sp., Erwinia sp., Serratia sp., Providencia sp., Corynebacterium sp., and Brevibacterium sp.