This invention provides an attenuated virus which comprises a modified viral genome containing nucleotide substitutions engineered in multiple locations in the genome, wherein the substitutions introduce synonymous deoptimized codons into the genome. The instant attenuated virus may be used in a vaccine composition for inducing a protective immune response in a subject. The invention also provides a method of synthesizing the instant attenuated virus. Further, this invention further provides a method for preventing a subject from becoming afflicted with a virus-associated disease comprising administering to the subject a prophylactically effective dose of a vaccine composition comprising the instant attenuated virus.
申请公布号
EP2139515(A2)
申请公布日期
2010.01.06
申请号
EP20080799746
申请日期
2008.03.31
申请人
THE RESEARCH FOUNDATION OF THE STATE UNIVERSITY OF NEW YORK