发明名称 |
C-TERMINUS MODIFICATION METHOD, C-TERMINUS IMMOBILIZATION METHOD AND ANALYSIS METHOD FOR PROTEIN OR PEPTIDE |
摘要 |
The present invention provides a method for inexpensively, easily and efficiently modifying the C-terminus of a protein or peptide; a method for easily and reliably isolating a C-terminal peptide fragment of a protein or peptide; and a method for rapidly, accurately and reliably determining an amino acid sequence of a protein or peptide by using a mass spectroscope. A method comprising the step of adding a formylation reagent and a catalyst to a protein or peptide to convert a carboxyl group into an aldehyde group. A method comprising the step of reacting a nucleophilic reagent with the aldehyde group to modify the C-terminus of the protein or peptide. A method comprising the step of reacting a support having a nucleophilic group to immobilize the protein or peptide. A method comprising the step of fragmenting the immobilized protein or peptide, washing the support, and isolating the C-terminal peptide fragment from the support. A method comprising the step of subjecting the isolated C-terminal peptide fragment to mass spectrometry and determining an amino acid sequence.
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申请公布号 |
US2009325227(A1) |
申请公布日期 |
2009.12.31 |
申请号 |
US20060090747 |
申请日期 |
2006.10.23 |
申请人 |
SHIMADZU CORPORATION;NARA WOMEN'S UNIVERSITY;OSAKA UNIVERSITY |
发明人 |
NAKAZAWA TAKASHI;OKA MUTSUMI;NISHIDA KIMIKO;YAMAGUCHI MINORU;KUYAMA HIROKI;ANDO EIJI;UEYAMA NORIKAZU;OKAMURA TAKAAKI;TSUNASAWA SUSUMU |
分类号 |
C12P21/00;C07K1/107;G01N33/68 |
主分类号 |
C12P21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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