发明名称 |
METHODS FOR PRODUCING INDUCIBLE AND/OR REPRESSIBLE EXPRESSION ACTIVE LINEAR RNA INTERFERENCE CASSETTES AND INDUCIBLE AND/OR REPRESSIBLE EXPRESSION ACTIVE LINEAR GENE CASSETTES AND THEIR USES |
摘要 |
The present invention relates to a (first) method for producing inducible and/or repressible expression active linear RNA interference constructs comprising a PCR amplification of a source polynucleotide comprising the inhibitory RNA coding sequence of interest or comprising a PCR amplification of a DNA source comprising a promoter using a reverse primer comprising the inhibitory RNA coding sequence of interest. The present invention furthermore relates to a (second) method for producing inducible and/or repressible expression active linear gene constructs comprising a PCR amplification of a source expression polynucleotide comprising a promoter sequence and the DNA sequence of interest or comprising a PCR amplification using the DNA sequence as a template. The present invention furthermore relates to libraries, arrays, cells and cell lines and kits utilizing the inducible and/or repressible expression active linear RNA interference constructs or the inducible and/or repressible expression active linear gene constructs. |
申请公布号 |
WO2009156030(A1) |
申请公布日期 |
2009.12.30 |
申请号 |
WO2009EP03686 |
申请日期 |
2009.05.25 |
申请人 |
KING FAISAL SPECIALIST HOSPITAL & RESEARCH CENTRE;TERRAMARK MARKENCREATION GMBH;KHABAR, KHALID, S., ABU |
发明人 |
KHABAR, KHALID, S., ABU |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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