发明名称 |
STANDARD MOLECULE FOR USE IN REAL-TIME PCR TEST, AND DETECTION OF THE STANDARD MOLECULE |
摘要 |
<p>Disclosed are: a standard molecule characterized in that the presence of a false-positive result caused by unintended contamination of a reagent, a sample or the like with the standard molecule can be confirmed simultaneously with the determination of the test results in real-time PCR test; a primer; a probe; and a test method using the standard molecule, the primer and the probe. In a real-time PCR test, the presence of a false-positive result caused by contamination with a standard molecule for a real-time PCR test can be determined simultaneously by carrying out the test using the standard molecule and a primer/probe, wherein the standard molecule contains both an artificial DNA sequence and a target region of DNA to be tested, wherein the artificial DNA sequence can be amplified under the same PCR conditions and in the same reaction vessel as those for the target region of the DNA to be tested in the real-time PCR test, and wherein the primer/probe is one used for amplifying/detecting only the DNA sequence.</p> |
申请公布号 |
WO2009157465(A1) |
申请公布日期 |
2009.12.30 |
申请号 |
WO2009JP61459 |
申请日期 |
2009.06.24 |
申请人 |
ORIENTAL YEAST CO., LTD.;YANO, TAKEO;SAKAI, YUMIKO;KURIHARA, TAKASHI |
发明人 |
YANO, TAKEO;SAKAI, YUMIKO;KURIHARA, TAKASHI |
分类号 |
C12Q1/68;C12N15/09 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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