| 摘要 |
FIELD: biotechnologies. ^ SUBSTANCE: invention is related to biotechnology and may be used for site-specific hydrolysis of DNA, which contains C5-methylcytosin bases. Strain Paracoccus carotinifaciens 3K is extracted from soil, which provides for production of site-specific endonuclease, which recognises and splits both chains of nucleotide sequence of DNA containing four C5-methylcytosin bases in site of recognition 5'-WCGNNNNNNNCGW-3', with production of single-nucleotide 3'-protruding ends. ^ EFFECT: invention makes it possible to produce new site-specific endonuclease Pcsl, which may be used for detection and analysis of methylated DNA. ^ 2 dwg, 3 ex |
