| 摘要 |
FIELD: medicine. ^ SUBSTANCE: invention refers to medicine, namely to vascular surgery, and can be used in treating the patients suffering from lower limb ischemia. That is ensured by instrumental preexamination to detect the affected region. Then palpation under spinal anaesthesia is used to localise posterior iliac crest, and along posterior iliac crest, a medical needle for bone marrow biopsy equipped with a mandrin and lateral apertures at its end is used for trepanopunctures of spongy substance of bone at pitch 1 cm and bone penetration depth 1-2 cm. Each puncture involves aspiration of bone marrow in amount 5-6 ml with a syringe 10 ml containing 0.5 ml of prepared mixture containing 0.9% NaCl 200 ml and heparin 5 ml. The total amount of the aspirated bone marrow is 400-600 ml. After each puncture the dissolved bone marrow is filtered and collected in a sterile air-tight package containing 43 ml of anticoagulant CPDA thereafter separated to produce mononuclear cells in amount 45-55 ml. To mark the points of cell material introduction, points in number 60 pcs at one injection per 4 cm2 of limb surface are drawn on shin and foot surface with a highlighter. Introduction is performed by intramuscular injections in the marked points in amount 1 ml at a depth 2-2.5 cm. ^ EFFECT: method allows improving clinical effectiveness of lower limb ischemia, including critical one, owing to intensified activity of local neoangiogenesis caused by release of introduced mononuclear cells of various growth factors and a number of cytokines. ^ 2 ex |
