Method of purifying APO-2 Ligand/trail using crystallisation in the cold

摘要

Disclosed is a method of recovering Apo2L/TRAIL from a mixture comprising (a) loading the mixture on a cation exchange column; (b) washing the cation exchange column with an equilibration buffer whereby non-binding components present in the mixture are removed; (c) eluting Ap02L1TRAIL bound to the cation exchange column with an elution buffer; (d) gradually cooling the eluate to a temperature of about 2 to 4C, whereby Apo2/TRAIL is spontaneously precipitated in a crystalline form to yield a mixture of mother liquor and Apo2L/TRAIL crystals, and (e) recovering Apo2L/TRAIL from the mixture obtained in step (d) in a purity of at least about 99%.