| 摘要 |
Disclosed is a method of screening a library of molecules to identify or select one or more members thereof which are candidate binding partners for one or more target entities comprising: (a) contacting an expression display library with one or more target entities; (b) subjecting said target entities to at least one washing step; (c) separating target entities which have become bound to one or more members of the expression library from unbound members of the expression display library by separation through an organic phase, thereby separating candidate binding partners for said target entities from other library members, wherein said target entity is a cell surface molecule on a cell or a molecule which is attached to a solid phase. In particular the expression display library may comprise scFv antibodies.
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