| 摘要 |
Provided is a method of detecting a nucleic acid sequence in a genomic sample, said method comprising providing the genomic sample comprising a quantity of duplex nucleic acids containing target nucleic acid sequences; providing a quantity of single- and/or double-stranded probes comprising probe nucleic acid sequences; providing a hybridization mixture comprising the genomic sample, the quantity of probes, a quantity of hybridization promoting agents and labels; incubating the hybridization mixture to provide an incubated mixture comprising complexes of the duplex nucleic acids, the probes and the labels; irradiating the incubated mixture with radiation effective to stimulate at least some of the labels to emit energy; and detecting from a fluorescent signal whether the probe nucleic acid sequences perfectly match the target nucleic acid sequence, to thereby detect whether the nucleic acid sequence is present in the genomic sample, wherein a ratio of the quantity of probes to the quantity of duplex nucleic acids is at least 109 , and the method is conducted without denaturing the duplex nucleic acids and without pcR amplification of the duplex nucleic acids. The method is based on triplex and quadruplex formation in the assay. Further provided are corresponding kits for using the assay. |
