| 摘要 |
PROBLEM TO BE SOLVED: To provide an improved medium for culturing Clostridium histolyticum and culture supernatants for biotechnologically producing collagenase enzymes. SOLUTION: The culture media contain water, fish gelatin and peptones derived from a non-mammalian source. The media preferably contain peptones derived from plants as a non-mammalian source. The media also may contain two or more different peptones. Disclosed are sterilized compositions comprising the media, a culture supernatant containing the collagenase of Clostridium histolyticum, a method for producing the culture supernatant, and a method for producing the collagenase. COPYRIGHT: (C)2010,JPO&INPIT |
